Mouse early diabetic nephropathy LC-MS/MS analysis

The present study aims to evaluate the alterations induced by type I diabetes and the associated hyperglycemia on the proteome of renal tissue using a transgenic experimental animal model. Diabetic and non-diabetic kidney samples were analyzed by liquid nano-chromatography mass spectrometry and protein abundance was evaluated bylabel free quantification.本研究旨在采用转基因实验动物模型，评估1型糖尿病（type I diabetes）及其伴发的高血糖症对肾组织蛋白质组（proteome）造成的改变。研究采用液相纳米色谱-质谱联用技术对糖尿病组与非糖尿病组的肾脏样本进行分析，并通过无标记定量（label free quantification）法评估蛋白质丰度。