Differentially Expressed Genes of Pseudomonas aeruginosa Isolates from Eyes with Keratitis and Healthy Conjunctival Sacs

P. aeruginosa isolates from keratitis and healthy conjunctival sacs were obtained. The transcriptome profile of P. aeruginosa was characterized by a high throughput RNA-seq strategy using the Illumina HiSeq 2500 platform. The DEGs were analyzed with DESeq and validated through quantitative real-time polymerase chain reaction (PCR) and with experimental mice. RNA-seq analysis of P. aeruginosa from keratitis and healthy conjunctival sacs

本研究收集了来自角膜炎患者与健康人结膜囊的铜绿假单胞菌（P. aeruginosa）分离株。采用Illumina HiSeq 2500平台开展高通量RNA测序（RNA-seq）以解析该菌的转录组特征。通过DESeq软件分析差异表达基因（Differentially Expressed Genes, DEGs），并借助实时定量聚合酶链式反应（PCR）及小鼠实验对其进行验证。针对源自角膜炎病例与健康人结膜囊的铜绿假单胞菌的RNA-seq分析