ATAC-seq in hippocampal GABAergic cells of SMARCA3 cKO mice

Purpose: To identify chromatin accessibility changes in CCK hippocampal interneurons from SMARCA3 cKO mice Method: Genetically expressing GFP nuclei were sorted from CCK+ or GAD2+ cells from WT or SMARCA3 cKO mice, and were used for ATAC seq using Nextseq sequencer. Results: Biostatistical analysis identified 3552 genes that were altered by SMARCA3 cKO in CCK cells. 6 hippocampi were pooled for one ATAC sample. We used mice that were CCK cre+/GAD2 cre+ crossed with L10-EGFP+ mice and SMARCA3 fl/fl and littermates without SMARCA3 fl/fl controls.

### 研究目的
鉴定SMARCA3条件性敲除（conditional knockout, cKO）小鼠胆囊收缩素（Cholecystokinin, CCK）阳性海马中间神经元的染色质可及性变化。
### 实验方法
从野生型（Wild Type, WT）或SMARCA3 cKO小鼠的CCK+或谷氨酸脱羧酶2（Glutamic Acid Decarboxylase 2, GAD2）阳性细胞中分选表达绿色荧光蛋白（Green Fluorescent Protein, GFP）的细胞核，使用Nextseq测序仪开展转座酶可及性测序（ATAC-seq）。
### 实验结果
经生物统计学分析，在CCK阳性细胞中鉴定出3552个受SMARCA3 cKO调控的差异基因。每个ATAC测序样本需混合6个海马体；实验所用小鼠为CCK-Cre+/GAD2-Cre+与L10-EGFP+小鼠及SMARCA3 flox纯合（SMARCA3 fl/fl）小鼠的杂交子代，对照组为不携带SMARCA3 flox等位基因的同窝野生型小鼠。