Next Generation Sequencing Analysis of human hepatocellular cercinoma cells HLF single cultured and HLF co-cultured with CD4+ T cells isolated from healthy donors

In this study, we investigated the role of CD40 in liver cancer progression. We elucidated the complex interplay between CD40 presenting in cancer cells with their ligand in T cells, and its interaction driven the dowstream signalings of cancer cell proliferation and apoptosis pathways. Overall design: To investigate the change of liver cancer cell line - HLF before and after exposed to healthy donors 'CD4+ T cells We performed gene expression profiling analysis using data obtained from RNA-seq of 2 groups, in which HLF single cultrure served as control group and HLF-T cells co-culture sereved as treatment group

本研究探讨了CD40在肝癌进展中的作用。我们阐明了癌细胞表面表达的CD40与其在T细胞上的配体之间的复杂相互作用，且该相互作用可驱动癌细胞增殖与凋亡通路的下游信号转导。总体实验设计：为探究肝癌细胞系HLF在与健康供体CD4+ T细胞共培养前后的基因表达变化，我们利用两组样本的RNA测序（RNA-seq）数据开展了基因表达谱分析，其中单培养HLF细胞作为对照组，HLF与T细胞共培养组作为处理组。