The mRNA-lncRNA-circRNA network profiling of Turner syndrome patient-derived induced pluripotent stem cells and their derived cardiomyocytes. The mRNA-lncRNA-circRNA network profiling of Turner syndrome patient-derived induced pluripotent stem cells and their derived cardiomyocytes

Heart deformity is the leading cause of mortality in Turner syndrome (TS) patients. To investigate the dysregulated ceRNA network in cardiomyocytes (CMs) of TS patients, we employed a ceRNA microarray to profile the mRNA-lncRNA-circRNA network in induced pluripotent stem cells (iPSCs) and their derived CMs from healthy donors (WT) and TS patients. Overall design: 3 WT and 3 TS patient-derived iPSC lines were generated from PBMCs. CMs were derived from these iPSC lines, respectively. The mRNA-lncRNA-circRNA networks in the WT and TS-derived iPSC and CM lines were examined with ceRNA microarrays that were capable of profiling 18k mRNAs, 68k lncRNAs and 88k circRNAs.

心脏畸形是特纳综合征（Turner syndrome，TS）患者的首要致死原因。为探究特纳综合征患者心肌细胞（cardiomyocytes，CMs）中失调的内源竞争RNA（competing endogenous RNA，ceRNA）网络，我们采用ceRNA芯片，对健康供体野生型（WT）及特纳综合征患者来源的诱导多能干细胞（induced pluripotent stem cells，iPSCs）及其分化得到的心肌细胞中的mRNA-lncRNA-circRNA网络进行表达谱分析。实验整体设计：从外周血单个核细胞（peripheral blood mononuclear cell，PBMC）中构建了3株野生型及3株特纳综合征患者来源的诱导多能干细胞系。分别以这些诱导多能干细胞系为基础分化得到心肌细胞。采用可同时检测18k条mRNA、68k条lncRNA及88k条circRNA的ceRNA芯片，对野生型及特纳综合征患者来源的诱导多能干细胞和心肌细胞系中的mRNA-lncRNA-circRNA网络进行了检测分析。