Identification of AR-responsive proteins in L. formosana.

The seedlings were treated with AR (pH 3.0) for one month. The pH of AR solution was adjusted with a mixture of H2SO4 and HNO3 in the ratio of 5∶1. The final concentration of H2SO4 and HNO3 were 0.45 and 0.09 mM, respectively.aSpot No. is the unique differentially expressed protein spot number. L, protein spot in L. formosana gel. b Database accession numbers according to NCBInr. c Description of the proteins identified by MALDI-TOF MS. d Theoretical mass (kDa) and pI of identified proteins. e Experimental mass (kDa) and pI of identified proteins. f Amino acid sequence coverage for the identified proteins. g Number of the matched peptides and the total searched peptides. hMascot searched score against the database NCBInr. Protein score is −10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 73 are significant (p i Spot abundance change. D decreased abundance of proteins, U increased abundance of protein.