Identification of AR-responsive proteins in L. formosana.

The seedlings were treated with AR (pH 3.0) for one month. The pH of AR solution was adjusted with a mixture of H2SO4 and HNO3 in the ratio of 5∶1. The final concentration of H2SO4 and HNO3 were 0.45 and 0.09 mM, respectively.aSpot No. is the unique differentially expressed protein spot number. L, protein spot in L. formosana gel. b Database accession numbers according to NCBInr. c Description of the proteins identified by MALDI-TOF MS. d Theoretical mass (kDa) and pI of identified proteins. e Experimental mass (kDa) and pI of identified proteins. f Amino acid sequence coverage for the identified proteins. g Number of the matched peptides and the total searched peptides. hMascot searched score against the database NCBInr. Protein score is −10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 73 are significant (p i Spot abundance change. D decreased abundance of proteins, U increased abundance of protein.供试幼苗经pH 3.0的酸性还原剂（AR）处理1个月。采用体积比5∶1的硫酸（H₂SO₄）与硝酸（HNO₃）混合液调节AR溶液的pH值，最终体系中硫酸和硝酸的浓度分别为0.45 mM和0.09 mM。 a 斑点编号：唯一的差异表达蛋白质斑点编号，L代表L. formosana凝胶中的蛋白质斑点。 b 数据库登录号：对应NCBInr数据库的登录号。 c 蛋白质鉴定描述：经基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定的蛋白质相关说明。 d 理论分子量与等电点：鉴定蛋白质的理论分子量（单位：kDa）及等电点（pI）。 e 实测分子量与等电点：鉴定蛋白质的实际分子量（单位：kDa）及等电点（pI）。 f 氨基酸序列覆盖率：鉴定蛋白质的氨基酸序列覆盖比例。 g 匹配肽段与总检索肽段数：匹配成功的肽段数量与总检索肽段数量。 h Mascot检索得分：基于NCBInr数据库的Mascot检索得分。蛋白质得分计算公式为−10×Log(P)，其中P为观测匹配结果为随机事件的概率；当蛋白质得分高于73时，差异具有统计学显著性（p < 0.05）。 i 斑点丰度变化：D表示蛋白质丰度下调，U表示蛋白质丰度上调。