Data_Sheet_1_Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing.docx

The sustainability and economic feasibility of modern biorefinery depend on the efficient processing of both carbohydrate and lignin fractions for value-added products. By mimicking the biomass degradation process in white-rote fungi, a tailored two-step fractionation process was developed to maximize the sugar release from switchgrass biomass and to optimize the lignin processability for bioconversion. Biomimicking biomass processing using Formic Acid: Fenton: Organosolv (F2O) and achieved high processability for both carbohydrate and lignin. Specifically, switchgrass pretreated by the F2O process had 99.6% of the theoretical yield for glucose release. The fractionated lignin was also readily processable by fermentation via Rhodococcus opacus PD630 with a lipid yield of 1.16 g/L. Scanning electron microscope analysis confirmed the fragmentation of switchgrass fiber and the cell wall deconstruction by the F2O process. 2D-HSQC NMR further revealed the cleavage of aryl ether linkages (β-O-4) in lignin components. These results revealed the mechanisms for efficient sugar release and lignin bioconversion. The F2O process demonstrated effective mimicking of natural biomass utilization system and paved a new path for improving the lignin and carbohydrate processability in next generation lignocellulosic biorefinery.

现代生物精炼厂的可持续性和经济可行性取决于对碳水化合物和木质素两相的高效加工，以实现增值产品的生产。通过模拟白腐真菌的生物降解过程，开发了一种定制化的两步分离工艺，旨在最大化 switchgrass 生物量中的糖释放，并优化木质素的生物转化加工性能。采用蚁酸：芬顿：有机溶剂（F2O）的生物模拟生物质加工方法，实现了碳水化合物和木质素的高加工性能。具体而言，经 F2O 处理的 switchgrass 预处理样品，其葡萄糖释放的理论产量达到了 99.6%。分离得到的木质素也易于通过 Rhodococcus opacus PD630 发酵，产生 1.16 g/L 的脂质。扫描电子显微镜分析证实了 F2O 过程对 switchgrass 纤维和细胞壁的破碎作用。二维 HSQC NMR 进一步揭示了木质素组分中芳醚键（β-O-4）的断裂。这些结果揭示了高效糖释放和木质素生物转化的机制。F2O 过程有效地模拟了自然生物质利用系统，为下一代木质纤维素生物精炼厂中木质素和碳水化合物的加工性能提升开辟了新的途径。