Data_Sheet_2_Genomic Perspectives on Aeromonas salmonicida subsp. salmonicida Strain 890054 as a Model System for Pathogenicity Studies and Mitigation of Fish Infections.CSV

Aeromonas salmonicida subsp. salmonicida is a Gram-negative bacterium that causes furunculosis, a fish disease claiming substantial economic losses in the aquaculture industry. Major challenges exist in monitoring and controlling fish infections in aquaculture farms. Development of management practices to improve the sustainability of fish farming with disease prevention necessitates studies using well-defined systems and well-characterized bacterial isolates. Even though several A. salmonicida subsp. salmonicida genomes have been completely assembled and thoroughly annotated, in vivo pathogenicity data are lacking. Here we present A. salmonicida subsp. salmonicida 890054 as a prototype strain for standardized furunculosis challenges with survival data. Computational analysis of sequencing results provided a complete circular genome with annotations of plasmids carrying virulence factors, antimicrobial resistance, and secondary metabolite coding genes. The analysis also revealed the presence of an IncU plasmid distinct from other IncU plasmids previously associated with Aeromonas.

杀鲑气单胞菌杀鲑亚种（Aeromonas salmonicida subsp. salmonicida）是一种革兰氏阴性菌（Gram-negative bacterium），可引发疖疮病（furunculosis）——一种给水产养殖业造成巨额经济损失的鱼类病害。当前水产养殖养殖场的鱼类感染监测与防控仍面临诸多重大挑战。为通过病害防控提升水产养殖的可持续性，制定配套管理措施，亟需采用定义明确的实验体系与经过充分鉴定的细菌分离株开展相关研究。尽管目前已有多株杀鲑气单胞菌杀鲑亚种的基因组完成全基因组组装并得到详尽注释，但仍缺乏体内致病性数据。本研究报道杀鲑气单胞菌杀鲑亚种890054，作为开展标准化疖疮病攻毒实验并附带存活数据的模式菌株。对测序结果的生物信息学分析获得了一条完整的环状基因组，并注释到携带毒力因子（virulence factors）、抗菌耐药（antimicrobial resistance）相关基因与次级代谢产物编码基因（secondary metabolite coding genes）的质粒。该分析还发现存在一种IncU质粒（IncU plasmid），其与此前报道的与气单胞菌属（Aeromonas）相关的其他IncU质粒均存在差异。