Tanshinone I inhibit normal and malignant hematopoiesis by repressing EZH2 activity [zebrafish]

Purpose: To explore the mechanism of how Tan I inhibits malignant hematopoiesis at the gene expression level Methods: 72 hpf Tg(c-mybhyper: GFP) zebrafish embryos treated with DMSO or Tan I 60 µM were collected for RNA sequencing Results: We found that 1882 genes were differentially expressed between DMSO and Tan I treatment in c-mybhyper fish embryos (p value <=0.05, |log2 fold change| >= 0.25) Conclusions: We identified MMP9 and ABCG2 as two possible downstream genes of Tan I's effects on EZH2 Overall design: 72 hpf Tg(c-mybhyper: GFP) zebrafish embryos treated with DMSO or Tan I 60 µM

研究目的：在基因表达层面探究丹参酮I（Tan I）抑制恶性造血的作用机制。
实验方法：收集经二甲基亚砜（DMSO）或60 μM丹参酮I（Tan I）处理的72 hpf Tg(c-mybhyper: GFP)转基因斑马鱼胚胎，开展RNA测序。
实验结果：在c-mybhyper转基因斑马鱼胚胎中，二甲基亚砜处理组与丹参酮I处理组间存在1882个差异表达基因（P值≤0.05，|log₂折叠变化|≥0.25）。
实验结论：本研究鉴定出基质金属蛋白酶9（MMP9）与ATP结合盒转运蛋白G2（ABCG2）为丹参酮I通过调控zeste同源物增强子2（EZH2）发挥生物学效应的两个潜在下游靶基因。
实验整体设计：以经二甲基亚砜（DMSO）或60 μM丹参酮I（Tan I）处理的72 hpf Tg(c-mybhyper: GFP)转基因斑马鱼胚胎为实验对象。