Pennisetum sinese Roxb plantation and organic manure alter bacterial and fungal communities structure in an ecological agriculture farm

We hypothesized that the soil microbial communities response strongly to the plantation of P. sinese and application of livestock manures, and show a significant correlation of soil microbial community compositions with soil physicochemical parameters. Here, soils with a P. sinese cultivation and fertilization history of 2 year in an ecological farm were selected to investigate the community diversification of bacteria and fungi using 16S rRNA/ITS1 gene-based Illumina MiSeq sequencing.

本研究假设，象草（P. sinese）种植与畜禽粪便施用会对土壤微生物群落产生强烈响应，且土壤微生物群落组成与土壤理化参数间存在显著相关性。本研究选取某生态农场中具有2年象草种植与施肥历史的土壤样品，采用基于16S rRNA/ITS1基因的Illumina MiSeq测序技术，探究细菌与真菌的群落多样性特征。