Structure of the Nt domain of mouse NBCe1 by homology modeling

For the generation of the Nt model for NBCe1, a pair-wise alignment of the Nt region (residues 82-381) of mouse NBCe1-B (NCBI accession NP_061230.2) and the homologous portion of human NDCBE Nt (NCBI accession AAY79176) was generated by SWISS-MODEL. The overall identity is 54.55% between the two sequences. The alignment was used for structural modeling with the crystal structure of human NDCBE Nt (PDB ID: 5JHO) as the template. Structural assessment shows that the simulated model of NBCe1 Nt had QMEAN value −3.25, Cβ value −3.11, solvation value −0.62, torsion value −2.54, and scored 1.86 by using a MolProbity approach.为构建NBCe1的Nt（N末端）结构模型，我们通过SWISS-MODEL对小鼠NBCe1-B（NCBI登录号NP_061230.2）的Nt区域（残基82-381）与人源NDCBE Nt的同源区段（NCBI登录号AAY79176）进行了双序列比对。二者的整体序列一致性为54.55%。以此比对为基础，以人源NDCBE Nt的晶体结构（PDB ID: 5JHO）为模板开展结构建模。结构评估结果显示，NBCe1 Nt的模拟模型的QMEAN值为−3.25、Cβ值为−3.11、溶剂化值为−0.62、扭转角值为−2.54，且经MolProbity方法评分为1.86。