Mean(P2) C

After 3 days of treatment, plates were scanned with CCD flatbed scanners (EPSON Perfection V600 Photo, Seiko Epson, Nagano, Japan), and images used to quantify root parameters with FIJI, by using the tool ‘segmented line’ (Schindelin et al., 2012) as described in Ristova and Busch (2017). In particular, we quantified: primary root length on day 10 (P), growth rate of P after treatment (P2), branching zone or the length of P between the first and last visible lateral root (R), average lateral root length (LRL), and visible lateral root number (LR.No).

处理3天后，采用CCD平板扫描仪（EPSON Perfection V600 Photo，精工爱普生，日本长野）对培养平板进行扫描，随后借助FIJI软件并使用‘分段直线’工具（Schindelin等，2012）对扫描所得图像开展根系参数量化分析，具体方法参照Ristova与Busch（2017）的研究方案。具体而言，本研究量化了以下参数：第10天主根长度（primary root length, P）、处理后主根生长速率（P2）、分支区长度（即首个与最后一个可见侧根之间的主根段长度，记为R）、平均侧根长度（average lateral root length, LRL）以及可见侧根数量（visible lateral root number, LR.No）。