Genomic and transcriptomic determinants of response to neoadjuvant therapy in rectal cancer

We performed RNA-seq on pre-treatment endoscopic biopsies from 114 patients, including 97 patients treated with NAT that qualified for outcome analyses We then performed gene expression profiling analysis using data obtained from RNA-seq Comparative gene expression profiling analysis of RNA-seq data between CR/iCR patients and ssgsea analysis我们对114例患者的治疗前内镜活检标本进行了RNA测序（RNA-seq），其中97例接受新辅助治疗（NAT）的患者符合预后分析的纳入条件。随后，我们利用RNA测序获得的数据开展基因表达谱分析，并针对完全缓解/临床完全缓解（CR/iCR）患者的RNA测序数据完成对比基因表达谱分析及ssgsea分析。