Proteomic study of avocado seed tissues (testa, cotyledon, and embryo) during postharvest shelf life

In this study, we scrutinized by comparative proteomics using Tandem Mass Tags (TMT) couple to synchronous-precursor selection (SPS)-MS3 the dynamic of the proteome of specific tissues of avocado seeds (testa, cotyledon, and embryo) during postharvest shelf life. Our proteomics pipeline allows the identification of more proteins in testa compared to other tissues, and also, we could track the dynamic of more protein in this tissue during PSL. In general, proteins associated with stress, glycolic process, and starch metabolism were commonly identify in each tissue. In contracts, a drastic difference in protein translation, amino acid, and fatty acid metabolism was observed among tissues during PSL.

本研究采用串联质量标签（Tandem Mass Tags, TMT）结合同步前体选择-MS3（Synchronous-precursor selection, SPS-MS3）的比较蛋白质组学方法，对牛油果种子的不同组织（种皮（testa）、子叶（cotyledon）及胚（embryo））在采后货架期（postharvest shelf life, PSL）内的蛋白质组动态变化展开了系统分析。本研究的蛋白质组学分析流程，相较于其他组织，可在种皮中鉴定到更多蛋白质；同时，我们能够追踪该组织在采后货架期内更多蛋白质的动态变化趋势。总体而言，与胁迫响应、糖酵解过程及淀粉代谢相关的蛋白质在各组织中均有普遍检出。与之相反，在采后货架期内，不同组织间在蛋白质翻译、氨基酸代谢及脂肪酸代谢方面存在显著差异。