Metagenome assembled genomes co_8-Bin_21_1.fa

Co-assemblies were binned with CONCOCT within anvi’o v5.1 by clustering scaffolds 2500 bp or longer into metagenome assembled genomes (MAGs) and manually refining them within the anvi’o platform. Furthermore, dRep v2.3.2 was used to dereplicate MAGs and obtain representative genomes with flags ‘-comp 50 -con 10’ and default values.本研究中的宏基因组共组装体通过CONCOCT工具在anvi’o v5.1平台中完成分箱操作：先将长度2500 bp及以上的支架序列聚类为宏基因组组装基因组（metagenome assembled genomes，MAGs），随后在anvi’o平台内对其进行手动精细化校正。此外，采用dRep v2.3.2工具对MAGs进行去冗余处理，并通过设置参数标志"-comp 50 -con 10"、其余参数采用默认值的方式获取代表性基因组。