Expression data from Arabidopsis gapcp mutant treated with ABA

Glycolytic Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde 3-phospate to 1,3-bisphosphoglycerate by coupling with the reduction of NAD+ to NADH. We generated mutants of the Arabidopsis plastidial GAPDH isoforms (At1g79530, At1g16300; GAPCp1, GAPCp2). gapcp double mutants (gapcp1 gapcp2) display a drastic phenotype of arrested root development and sterility.Complex interactions occurring between ABA and sugar signal transduction pathways have been shown, but the molecular mechanisms connecting both pathways are not well understood. Since we found drastic carbohydrate changes in gapcp1 gapcp2, we studied their response to ABA. by performing a microarray analysis comparing gapcp1 gapcp2 and wild type seedlings after a long term treatment with ABA. 15-day old Arabidopsis seedlings (Col 0) and gapcp1gapcp2 double mutants were used for RNA extraction and hybridization on Affymetrix microarrays.

糖酵解型甘油醛-3-磷酸脱氢酶（Glycolytic Glyceraldehyde 3-phosphate dehydrogenase, GAPDH）可催化甘油醛-3-磷酸转化为1,3-二磷酸甘油酸，并偶联NAD+还原为NADH的反应。本研究构建了拟南芥质体型GAPDH同工型的突变体，对应基因座分别为At1g79530、At1g16300，命名为GAPCp1、GAPCp2。gapcp双突变体（gapcp1 gapcp2）呈现出根系发育停滞与育性完全丧失的极端表型。
已有研究证实，脱落酸（Abscisic Acid, ABA）与糖类信号转导通路之间存在复杂的交互调控，但连接二者的分子机制尚未被充分阐明。鉴于我们在gapcp1 gapcp2双突变体中观测到显著的碳水化合物代谢变化，我们通过芯片分析技术，对比了经长期ABA处理后的gapcp1 gapcp2双突变体与野生型幼苗的基因表达谱。本实验以15日龄的拟南芥Col-0野生型幼苗及gapcp1gapcp2双突变体为材料，提取总RNA后在Affymetrix基因芯片上完成杂交实验。