CAPTURE-3C-seq analysis of locus-specific long-range DNA interactions in HeLa stable cells expressing LAMPS and sgHS2

We demonstrate that LAMPS-mediated LLPS induces the formation of long-range chromatin interactions Overall design: We combined the chromatin interaction assays (Chromatin Conformation Capture or 3C) with the high affinity dCas9 purification approach to identify locus-associated long-range interactions (CAPTURE-3C-seq).

我们证实，LAMPS介导的液-液相分离（liquid-liquid phase separation, LLPS）可诱导远距离染色质相互作用的形成。总体实验设计：我们将染色质相互作用检测技术（染色质构象捕获技术，即3C）与高亲和力dCas9纯化方法相结合，用以鉴定位点相关的远距离相互作用（CAPTURE-3C-seq）。