Single-cell sequencing of sorted hepatocytes isolated from livers naïve and LCMV-infected (2 days) C57Bl/6J mice

Purpose: Elucidate the hepatocyte-specific effects of innate antiviral response to a hepatotropic virus. Methods: Hepatocytes were isolated using a standard 2 step perfusion protocol and viable CD45 negative single cells were sorted on a Sony SH800 instrument und subsequently processed using a 10x Genomics Controller according to standard protocols. Results: Hepatocytes upregulate inflammatory genes and downregulate metabolic genes. Conclusion: Hepatocytes are important innate immune signaling platforms in the liver during viral infection. Overall design: sorted hepatocytes were processed with the 10X Genomics Chromium Single Cell and sequenced on the Illumina HiSeq 4000

研究目的：阐明针对嗜肝病毒（hepatotropic virus）的固有抗病毒应答（innate antiviral response）在肝细胞中的特异性效应。
实验方法：采用标准两步灌流方案分离肝细胞，通过Sony SH800仪器分选存活的CD45阴性单细胞，随后依照标准流程使用10x Genomics控制器完成样本处理。
实验结果：肝细胞可上调炎症基因的表达，并下调代谢基因的表达。
研究结论：病毒感染期间，肝细胞是肝脏内重要的固有免疫信号转导平台。
整体实验设计：分选所得肝细胞采用10x Genomics Chromium单细胞系统进行处理，并在Illumina HiSeq 4000测序平台上完成测序。