Performance of male IL-1R1^−/− mice versus male WT on a battery of tasks.

(a) Visuo-spatial reference memory was assessed using the Y-maze across 20 trials (n = 5 per trial block) in male IL-1R1−/− (n = 11) compared to male WT mice (n = 9). (b) The time spent freezing before (too small to be clearly visible), and 48 hours following, 0.4 mA foot shock for male IL-1R1−/− mice and WT mice in the fear conditioning paradigm. (c) The number of squares crossed in 3 minutes in the open field and (d) the number of rears recorded in open field, across 3 minutes (e) the time spent in open arms of the elevated plus maze and (f) entries into open arms as a proportion of total arm entries (for male and females combined). Data are expressed as mean ± SEM and figure (a, b) were analysed by two way repeated-measures ANOVA with strain as between subjects factor and time/block as within subjects factor and all other figures were analysed by one-tailed t-test and significant differences are denoted by ** p<0.001 and *p<0.05.(a) 采用Y型迷宫（Y-maze）评估视觉空间参考记忆（Visuo-spatial reference memory），实验共开展20轮，每试验区块纳入5只小鼠；实验对象为11只雄性IL-1受体1基因敲除（IL-1R1−/−）小鼠，并以9只雄性野生型（Wild Type, WT）小鼠作为对照。(b) 在恐惧条件反射范式（fear conditioning paradigm）中，记录雄性IL-1R1−/−小鼠与野生型小鼠在0.4mA足部电击前（时长过短无法清晰显示）及电击后48小时的僵滞时间。(c) 旷场实验（open field）中3分钟内小鼠穿越的方格数，(d) 旷场实验3分钟内记录的小鼠直立行为次数；(e) 高架十字迷宫（elevated plus maze）中小鼠在开放臂的停留时间，以及(f) 开放臂进入次数占总臂进入次数的比例（实验涵盖雌雄小鼠）。所有数据均以平均值±标准误（Standard Error of the Mean, SEM）表示；其中图(a)、(b)采用双因素重复测量方差分析，以品系为组间因素、时间/区块为组内因素；其余图表均通过单尾t检验进行统计分析，显著性差异标注为**p<0.001与*p<0.05。