Androgen receptor-coregulators hormone responsiveness LNCaP / GSF

The text description is: LNCaP are androgen receptor expressing prostate carcinoma cells. Genital skin fibroblasts also express the androgen receptor. Cells were either proliferating or they were G0-arrested. Treatment of cells was performed with either the androgen DHT (dihydrotestosterone), the androgen analogue R1881 (methyltrienolone), or the solvent ethanol. Some hybridizations were performed in the type 1 design. In these cases, the hormone treated sample was hybridized against the ethanol treated sample on the same microarray. Hormone mediated induction or repression of gene transcription can directly be deduced from R/G normalized ratios on arrays. For other experiments, the ethanol treated control and the hormone treated experiment were hybridized on separate microarrays against a common reference of RNA. This reference was composed out of common reference batch CRG (50%) and a fibroblast RNA (50%). This reference was called mixed reference. For all experiments, the same batch of mixed reference was used. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Ethanol, R1881 (methyltrienolone), or DHT (dihydrotestosterone) Cell Line: LNCaP (prostate cancer) or foreskin fibroblasts Culture Synchrony: proliferation / Go-arrest Keywords: compound_treatment_design Computed

本数据集涉及的细胞包括：表达雄激素受体（androgen receptor）的LNCaP前列腺癌细胞，以及同样表达雄激素受体的生殖器皮肤成纤维细胞。所培养的细胞分为两种状态：增殖状态与G0期阻滞（G0-arrested）状态。细胞分别经雄激素双氢睾酮（dihydrotestosterone, DHT）、雄激素类似物甲基曲诺酮（methyltrienolone, R1881）或溶剂乙醇处理。部分杂交实验采用1型设计：将激素处理样品与乙醇处理样品在同一张微阵列（microarray）上进行杂交，此时可通过芯片的R/G归一化比值直接推导激素介导的基因转录上调或下调情况。其余实验中，乙醇处理的对照组与激素处理的实验组分别与通用参考RNA在独立的微阵列上完成杂交。该通用参考样本由50%的CRG通用参考批次与50%的成纤维细胞RNA混合制备，被称为混合参考样本（mixed reference），所有实验均使用同一批次的该混合参考样本。化合物处理实验设计指对化合物或化学物质（包括激素这类生物活性化合物）给药后的细胞应答进行检测的实验类型。本数据集的化合物处理方案包含乙醇、R1881（甲基曲诺酮）及DHT（双氢睾酮）；所用细胞系为LNCaP（前列腺癌）细胞或包皮成纤维细胞；培养同步状态为增殖/G0期阻滞。关键词：化合物处理实验设计（compound_treatment_design），经计算