The histone lysine acetyltransferase HBO1 (KAT7) regulates hematopoietic stem cell quiescence and self-renewal. The histone lysine acetyltransferase HBO1 (KAT7) regulates hematopoietic stem cell quiescence and self-renewal

In this study, we have used inducible and tissue-specific genetic deletion to investigate the function of HBO1 in the hematopoietic system. RNA-seq was used to examine the dependence of gene expression on the presence of HBO1(KAT7). Two different conditional expression sytems were used to induce Cre recombinase and delete a floxed allele of HBO1 in this study. This was to control for the treatement effect during Cre induction (interferon induction of Mx1-cre vs. tamoxifen induction of Rosa26-CreERT2). This data set indicates that HBO1 promotes expression of multiple genes encoding a hematopoietic transcription factor network essential for HSC quiescence and self-renewal in adult hematopoiesis. Overall design: 4 induced homozygous mutant vs. 4 induced heterozygous controls per specific cre-recombinase and per cell type (2 methods of deletion, 2 cells populations) => (4+4)x2x2=32 samples

本研究采用诱导型及组织特异性基因敲除技术，探究HBO1在造血系统中的生物学功能。本研究通过RNA-seq技术，检测基因表达对HBO1（KAT7）存在与否的依赖性。本研究使用两种不同的条件性表达系统，诱导Cre重组酶并敲除HBO1的floxed等位基因，此举旨在控制Cre诱导过程中的处理效应：即干扰素诱导的Mx1-cre与他莫昔芬诱导的Rosa26-CreERT2两种诱导方式的差异。本数据集显示，HBO1可促进多个基因的表达，这些基因编码的造血转录因子网络，对成体造血过程中造血干细胞（Hematopoietic Stem Cell, HSC）的静息态维持与自我更新至关重要。实验整体设计：针对每一种Cre重组酶系统及每一种细胞群（共2种敲除方法、2种细胞群），设置4例诱导型纯合突变样本与4例诱导型杂合对照样本，总样本量为(4+4)×2×2=32个。