Differential protein expression in berry skin from red grapes with varying hybrid character

Protein expression from berry skin of four different red grape biotypes was compared at a proteome-wide level by bottom-up shotgun proteomics, label free quantification and MaxQuant-assisted computational analysis. Red grapes were from a purebred Vitis vinifera (Aglianico cv.), a V. vinifera (local Sciascinoso cv.) grafted onto an American rootstock, an interspecific hybrid (V. vinifera × V. labrusca, Isabel) and an uncharacterized red grape with some hybrid lineage, as demonstrated by the presence of relatively high amounts of anthocyanidin 3,5-O-diglucosides. The aim was assessing the differences among red grape biotypes at a protein expression levels, also addressing the possible effect of the grafting on the phenotypic expression of some key metabolic enzymes in grape berries.

本数据集采用自下而上鸟枪法蛋白质组学（bottom-up shotgun proteomics）、无标记定量（label free quantification）以及MaxQuant辅助计算分析的方法，在全蛋白质组水平上比较了4种不同红葡萄生物型的果皮蛋白质表达谱。
供试红葡萄样本包括：纯种欧洲葡萄（Vitis vinifera）品种Aglianico、嫁接于美国砧木的本地欧洲葡萄（V. vinifera）品种Sciascinoso、种间杂交品种Isabel（V. vinifera × V. labrusca），以及一类带有部分杂交血统的未鉴定红葡萄——该类葡萄的标志性特征为含有相对较高含量的花青素3,5-O-二葡萄糖苷。
本研究旨在明确不同红葡萄生物型间的蛋白质表达差异，同时探讨嫁接对葡萄浆果中部分关键代谢酶表型表达的潜在影响。