Development and characterisation of a laboratory model of post streptococcal autoimmune cardiac and neurobehavioral complications - Dataset

In this dataset we have provided data on behavioural assessment on rats before and after exposure to streptococcal antigens, histological changes and antibody responses against host tissue proteins. Behavioural tests such as food manipulation, beam walking, grooming, marble burying and light/dark box tests were performed to assess the changes in fine motor coordination, gait and balance, obsessive-compulsive behaviour and anxiety like behaviour respectively before and after exposure to streptococcal antigens. Heart tissues were dissected from rats and stained with haematoxylin and eosin stain and scored them for infiltration of mononuclear cells in the myocardium and valvular tissues. ELISAs were performed on serum collected from rats to assess the antibody response against host tissue proteins following exposure to streptococcal antigens.

本数据集收录了大鼠暴露于链球菌抗原（streptococcal antigens）前后的行为学评估数据、组织学变化数据以及针对宿主组织蛋白的抗体应答相关数据。实验人员先后开展了多类行为学测试：食物操控实验、横梁行走实验、理毛行为实验、埋珠实验及明暗箱实验，分别用以评估大鼠暴露于链球菌抗原前后的精细运动协调能力、步态与平衡能力、强迫样行为及焦虑样行为的变化。研究者从大鼠体内摘取心脏组织，采用苏木精-伊红染色（haematoxylin and eosin stain）对组织进行染色，并对心肌组织与瓣膜组织中的单核细胞浸润程度进行评分。研究人员采集大鼠血清并开展酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay，ELISA，复数形式ELISAs），以评估大鼠暴露于链球菌抗原后针对宿主组织蛋白的抗体应答水平。