Ctenopharyngodon idella Raw sequence reads

To investigate the changes of the gut microbiota in grass carp (Ctenopharyngodon Idella), grass carps were administrated with different dosages of Bacillus subtilis spores (B.s-CotC-CsPmy)(1×105, 1×108, 1×1011 CFU/g pellets). And the fishes were named BL, BM, BH groups, respectively. Grass carp administrated with B.s-CotC (BC group) and without spores(NC group) were considered as control. 8 weeks after the beginning of the administration, three fishes in each group were euthanized as mentioned above and sampled. The whole intestines were aseptically excised and opened. They were rinsed with sterilized PBS to remove the contents. Intestines from three fishes in the same group were collected into one sterile centrifuge tube. The intestinal samples were homogenized with sterilized PBS. The genomic DNA was extracted using the OMEGA D3350 Bacterial DNA Kit (Omega, USA) according to manufacturer's protocols. The V3-V4 regions of 16S ribosomal DNA of bacteria were amplified, purified and pooled in equimolar and paired-end sequenced (2 × 250 bp) on an Illumina MiSeq platform

为探究草鱼（Ctenopharyngodon idella）肠道菌群的变化，本实验向草鱼投喂不同剂量的枯草芽孢杆菌孢子（B.s-CotC-CsPmy），剂量分别为1×10^5、1×10^8、1×10^11 CFU/g（菌落形成单位/克）颗粒饲料，并将三个剂量组分别命名为BL、BM、BH组。设置两组对照：投喂B.s-CotC的草鱼组（BC组）与未投喂孢子的空白对照组（NC组）。投喂开始8周后，每组选取3尾草鱼按照前述方法实施安乐死并采集样本。无菌剥离并剪开完整肠道，使用灭菌磷酸盐缓冲液（Phosphate Buffered Saline，PBS）冲洗以去除肠道内容物。将同组3尾草鱼的肠道样品收集至同一无菌离心管中，用灭菌PBS将肠道样品制备成匀浆。采用OMEGA D3350细菌DNA提取试剂盒（Omega，美国），依照制造商说明书提取基因组DNA。对细菌16S核糖体DNA的V3-V4可变区进行扩增、纯化，将扩增产物以等摩尔浓度混合后，在Illumina MiSeq平台上进行双末端测序（2×250 bp）