Dataset for: Nematode‐associated microbial taxa do not correlate with host phylogeny, geographic region or feeding morphology in marine sediment habitats

Marine nematode microbiomes were studied by isolating host-associated microbial taxa in nematodes from the Gulf of Mexico. A two‐gene metabarcoding approach was used to amplify the V4 region of the 16S ribosomal RNA (rRNA) gene targeting bacteria/archaea and the V1–V2 region of the 18S rRNA gene targeting microbial eukaryotes. This dataset contains nematode taxonomic identifications, video images, and 18S rRNA, 16S rRNA, NGS reads (fastq) from samples collected in the Gulf of Mexico in June and July of 2007. This dataset supports the publication: Schuelke, T., Pereira, T.J., Hardy, S.M., & Bik, H.M. (2018). Nematode-associated microbial taxa do not correlate with host phylogeny, geographic region or feeding morphology in marine sediment habitats. Molecular Ecology, 27(8): 1930-1951. doi: 10.1111/mec.14539

本研究通过分离墨西哥湾线虫体内的宿主相关微生物类群，对海洋线虫菌群展开探究。研究采用双基因元条形码（metabarcoding）技术，靶向扩增细菌/古菌的16S核糖体RNA（16S ribosomal RNA，rRNA）基因V4区，以及微生物真核生物的18S rRNA基因V1–V2区。本数据集包含2007年6月至7月于墨西哥湾采集的样本对应的线虫分类鉴定结果、视频影像，以及18S rRNA、16S rRNA测序数据与下一代测序（Next Generation Sequencing，NGS）fastq格式读数。本数据集支撑以下已发表论文：Schuelke T、Pereira TJ、Hardy SM、Bik HM（2018）《海洋沉积物生境中线虫关联微生物类群与宿主系统发育、地理区域及取食形态无显著关联》，《分子生态学》，27(8): 1930-1951. doi: 10.1111/mec.14539