Data_Sheet_1_Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets.PDF

Nezara viridula is a polyphagous stink bug that feeds on crops of economic importance such as corn, soybean and cotton. To increase understanding of the ability of this pest insect to feed on such diverse cropping systems, we analyzed the impact of an exclusive diet of corn or green bean on the enzymatic activity and transcriptomic profile of digestive enzymes. Growth rate and survival were reduced when insects were reared exclusively on green bean compared to corn. However, the overall protease and nuclease activity profiles were comparable between the two treatments. Distinct differences in inhibitor sensitivity and activity were seen in some cases, particularly for serine proteases in some regions of the midgut. The transcription profiles from N. viridula fed on corn versus green bean were distinct on principal component analysis of RNA-seq data. While specific transcripts differentially transcribed according to diet and across several tissues were identified, a large number of these transcripts remain unannotated. Further annotation for identification of these genes will be important for improved understanding of the remarkable polyphagy of N. viridula.

稻绿蝽（Nezara viridula）是一种多食性臭蝽，可取食玉米、大豆、棉花等具有经济重要性的作物。为深入理解该害虫取食此类多样化种植系统的能力，本研究分析了单一饲喂玉米或青豆对其消化酶活性与消化酶转录组谱的影响。与仅以玉米为食的试虫相比，全程单一饲喂青豆的试虫其生长速率与存活率均显著降低。但两类处理组的整体蛋白酶与核酸酶活性谱并无显著差异。不过在部分情形下，二者的抑制剂敏感性及酶活性存在明显差异，尤以中肠特定区域的丝氨酸蛋白酶最为突出。对取食玉米与青豆的稻绿蝽进行RNA测序（RNA-seq）数据的主成分分析（Principal Component Analysis）显示，二者的转录谱存在显著区别。本研究虽鉴定出一批随食性及不同组织而发生差异转录的特定转录本，但其中绝大多数仍未完成注释。对这些转录本对应的基因开展进一步注释，将有助于更深入地理解稻绿蝽出众的多食性特性。