Carcinus maenas Transcriptome. Carcinus maenas

RNA was isolated from 12 tissues of 4 Carcinus maenas individuals. For every tissue the RNA from each individual was pooled. The RNA was sequenced on an Illumina HiSeq 2500 sequencing machine (Exeter sequencing service: http://ess-wiki.exeter.ac.uk/ess/Exeter_Sequencing_Service). After quality control the RNA was used to produce a de novo assembled transcriptome. The transcriptome was annotated through sequence similarity searches to the NCBI databases. The immune pathways were characterized in detail to provide a basis for further studies.研究样本取自4只欧洲滨蟹（Carcinus maenas）个体的12种组织，从中提取总RNA。针对每一类组织，将不同个体来源的RNA进行混合。随后使用Illumina HiSeq 2500测序平台完成RNA测序，测序服务由埃克塞特测序服务中心提供（网址：http://ess-wiki.exeter.ac.uk/ess/Exeter_Sequencing_Service）。质控环节完成后，利用质控后的RNA开展从头转录组组装。通过将组装得到的转录组序列与美国国家生物技术信息中心（National Center for Biotechnology Information，NCBI）数据库进行相似性比对，完成转录组功能注释。本研究对免疫通路进行了详细解析，为后续相关研究奠定了基础。