Elements of ddLMS PCR typing systems for Acinetobacter sp.

anumber of bands in fingerprint pattern could be lower than the number of copies of the target sequence within genomic DNA. That happens when PCR products generated from different part of genomic DNA (upstream from different copies of the target sequence) have the same length and it cannot be separated by gel electrophoresis.Elements of ddLMS PCR typing systems for Acinetobacter sp.指纹图谱中的条带数目可能低于基因组DNA内靶序列（target sequence）的拷贝数。当从基因组DNA不同区域（即靶序列不同拷贝的上游区域）扩增得到的聚合酶链式反应（PCR）产物长度一致，且无法通过凝胶电泳（gel electrophoresis）进行分离时，即会发生此类现象。以上即为针对不动杆菌属（Acinetobacter sp.）的ddLMS PCR分型系统的构成要素。