Supplementary Material for: HPA1-29w genotyping and the foundation for the platelet apheresis registry in Jiangsu province of China by MassARRAY spectrometry

Introduction: This study aimed to investigate the allele frequencies of the human platelet antigens HPA-1-29w system in Jiangsu (China) and establish the platelet apheresis registry in blood donors. Methods: HPA genotyping was performed using the MassARRAY iPLEX® platform. Allele and genotype frequencies were estimated by direct counting and tested for Hardy–Weinberg equilibrium. The transfusion mismatch probability was calculated for every HPA specificity. Results: The HPA allele frequencies in the Jiangsu Han population of HPA-1b, -2b, -3b, -4b, -5b, -6b, -11b, -15b, and -21b were 0.0055, 0.0530, 0.4116, 0.0015, 0.0155, 0.0162, 0.0003, 0.5317, and 0.0070, respectively, in which a heterozygote of HPA-11a/b was first detected in China. Only allele a was detected for HPA-7-10w、-12-14w、-16-20w and -22-29w quasi-systems. The highest mismatch rate of HPA genes in 1640 platelet donors was the HPA-15 system, followed by the HPA-3 system with a rate of 37.4% and 36.71%, respectively. Conclusion: China’s largest-scale platelet registry of HPA-1-29w has been explored. The MassARRAY platform may help to found the platelet apheresis registry which would be useful to provide matching platelets and lead to a more accurate, effective, and safe transfusion for patients with platelet therapy.

研究背景：本研究旨在探究中国江苏地区人群人类血小板抗原（human platelet antigens，HPA）-1~29w系统的等位基因频率，并建立献血者血小板单采供者登记库。研究方法：采用MassARRAY iPLEX®平台开展HPA基因分型；通过直接计数法估算等位基因及基因型频率，并进行哈迪-温伯格平衡（Hardy–Weinberg equilibrium）检验；同时计算每一种HPA抗原特异性对应的输血不相容概率。研究结果：江苏汉族人群中，HPA-1b、-2b、-3b、-4b、-5b、-6b、-11b、-15b及-21b的等位基因频率分别为0.0055、0.0530、0.4116、0.0015、0.0155、0.0162、0.0003、0.5317及0.0070，其中国内首次检出HPA-11a/b杂合子；HPA-7~10w、-12~14w、-16~20w及-22~29w类抗原系统仅检出等位基因a。在1640名血小板供者中，HPA基因不相容率最高的为HPA-15系统，其次为HPA-3系统，不相容率分别为37.4%与36.71%。研究结论：本研究构建了国内目前规模最大的HPA-1~29w血小板单采供者登记库；MassARRAY平台可助力该类血小板单采供者登记库的建立，从而为血小板治疗患者提供匹配的血小板输注，实现更精准、高效且安全的输血治疗。