Additional file 9 of TGF-β1 dominates stromal fibroblast-mediated EMT via the FAP/VCAN axis in bladder cancer cells

Additional file 9: qRT-PCR (A, B), WB(C, D), and immunohistochemistry (E, F) showed that TGF-β1/FAP/VCAN axis regulates bladder cancer EMT in vivo. CAF has a stronger EMT-inducing effect than NF. Overexpression of FAP enhanced the EMT-inducing effects of stromal fibroblasts, while knockdown of FAP weakened those effects.补充材料9：实时荧光定量逆转录聚合酶链反应（quantitative reverse transcription PCR，qRT-PCR，A、B组）、蛋白质印迹法（Western Blot，WB，C、D组）以及免疫组织化学（immunohistochemistry，E、F组）结果证实，转化生长因子-β1（TGF-β1）/成纤维细胞活化蛋白（FAP）/Versican（VCAN）轴可在体内调控膀胱癌上皮间质转化（Epithelial-Mesenchymal Transition，EMT）。癌相关成纤维细胞（CAF）诱导上皮间质转化的效应强于正常成纤维细胞（NF）。过表达FAP可增强基质成纤维细胞的上皮间质转化诱导能力，而敲低FAP则会削弱该效应。