Gene expression profile at single cell level of fibroblasts from untreated (UT) and imiquimod (IMQ)-induced mouse skin.

We used single-cell RNA sequencing (scRNA-seq) to analyze the heterogeneity of dermal fibroblasts in homeostasis and skin inflammation. Dermal fibroblasts of the UT/IMQ-induced PdgfraDreER-tdTomato mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of tdTomato signal and analyzed using scRNA-seq.

本研究采用单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）技术，分析稳态与皮肤炎症状态下真皮成纤维细胞的异质性。本研究依据tdTomato信号的有无，通过荧光激活细胞分选术（Fluorescence-activated cell sorting, FACS）分离UT/IMQ诱导的PdgfraDreER-tdTomato小鼠的真皮成纤维细胞，并采用scRNA-seq完成测序分析。