Immunopeptidome analysis of 4T1 murine breast cancer cells.

This study aimed to identify Tumor Associated Antigens (TAA)s by Immunoaffinity purification of MHC peptides and LCMS analysis, verifying their immunogenicity and testing their tumor control potential in a highly aggressive preclinical model of triple-negative breast cancer in mice. Among the MHC peptides thus identified, an Endogenous Retroviral (ERV) peptide was also found, and this peptide, as well as several other peptides from TAAs, were shown to possess tumor control potential in a cancer vaccine setting in mice, by restricting tumor growth compared to controls.

本研究旨在通过免疫亲和纯化主要组织相容性复合体（Major Histocompatibility Complex, MHC）肽段并结合液相色谱-质谱联用（Liquid Chromatography-Mass Spectrometry, LC-MS）分析鉴定肿瘤相关抗原（Tumor Associated Antigens, TAA），同时验证其免疫原性，并在小鼠高侵袭性三阴性乳腺癌临床前模型中检测其肿瘤防控潜力。在所鉴定得到的MHC肽段中，同时发现了一种内源性逆转录病毒（Endogenous Retrovirus, ERV）肽段；该肽段与数种源自TAA的其他肽段，在小鼠癌症疫苗研究场景中被证实具有肿瘤防控潜力，相较于对照组可抑制肿瘤生长。