Structural studies of redox Nickel enzymes and nickel proteins

We are interested in the study of structure/function relationships of nickel enzymes, nickel proteins and nickel protein-based artificial metalloenzymes. Our objectives are the structural characterization of our artificial metalloenzymes as well as the trapping of reaction intermediates. We are alos interested in the biosynthesis of carbon monoxide dehydrogenase (CODH), a nickel enzyme with a complex multimetallic active site. We aim at the determination of the crystal structure of CODH and its nickel chaperones CooJ, CooT and CooC, involved in nickel insertion into CODH.

本研究聚焦镍酶、镍蛋白及基于镍蛋白的人工金属酶（artificial metalloenzymes）的结构与功能关系研究。本研究的核心目标包括对人工金属酶进行结构表征，以及捕获反应中间体。我们同时关注一氧化碳脱氢酶（carbon monoxide dehydrogenase, CODH）的生物合成——这是一类拥有复杂多金属活性中心的镍酶。本研究旨在解析一氧化碳脱氢酶（CODH），以及参与其镍插入过程的镍伴侣蛋白CooJ、CooT与CooC的晶体结构。