Profiling histone modifications in clinical archived formalin fixed paraffin embedded samples. Profiling histone modifications in clinical archived formalin fixed paraffin embedded samples

Here, we present FACT-Seq, a highly sensitive method to decode the histone modifications in FFPE tissues. The FACT-Seq generates the high-quality chromatin profiles from both active and silent histone modifications with 1000 nuclei purified from a single FFPE tissue section, and reveals the disease-specific super enhancers in the FFPE archived human colorectal and human glioblastoma multiforme cancer tissue. In summary, the approach allows decoding the histone modifications that regulate gene expression in archival FFPE tissues with high sensitivity, in order to better understand epigenetic regulation in cancer and precision medicine. Overall design: FACT-Seq of mouse FFPE kidney and clinical archived CRC and GBM.

本研究报道FACT-Seq技术，这是一种可解析福尔马林固定石蜡包埋（Formalin-Fixed Paraffin-Embedded, FFPE）组织组蛋白修饰的高灵敏度方法。该技术仅需从单张FFPE组织切片中纯化得到的1000个细胞核，即可获取覆盖活性与沉默型组蛋白修饰的高质量染色质图谱，并可在存档的人类结直肠癌与多形性胶质母细胞瘤FFPE组织中鉴定出疾病特异性超级增强子。综上，该方法可高灵敏度地解析存档FFPE组织中调控基因表达的组蛋白修饰，为深入解析癌症与精准医学领域的表观遗传调控机制提供支撑。实验整体设计：对小鼠FFPE肾脏组织以及临床存档的结直肠癌（Colorectal Cancer, CRC）与多形性胶质母细胞瘤（Glioblastoma Multiforme, GBM）样本开展FACT-Seq检测。