GSK3β as a potential regulator in AML: a pan-cancer multi-omics analysis

Description: This dataset contains all underlying data supporting the findings in the aforementioned manuscript. It encompasses: (1) Bioinformatics data: Normalized gene expression matrices for GSK3 isoforms across 31 TCGA cancer types and GTEx normal tissues, corresponding immune cell enrichment scores (calculated via ssGSEA using the Bindea et al. gene signatures), and processed survival analysis data (Cox regression results). (2) In vitro experimental data: Raw flow cytometry files (.fcs format) for apoptosis and cell cycle analysis of human AML THP-1 cells treated with the GSK3 inhibitor CHIR-99021 or DMSO control, and the original, uncropped Western blot images for proteins including c-Myc, and PARP1 (showing cleavage). The data were generated as part of a study to elucidate the pan-cancer landscape and therapeutic potential of GSK3β, with particular focus on acute myeloid leukemia (AML). These data can be reused for meta-analyses, computational validation, and comparative studies in oncology and hematology.数据集说明：本数据集包含支撑上述手稿研究发现的全部底层数据，具体涵盖以下内容： (1) 生物信息学数据：31种癌症基因组图谱（The Cancer Genome Atlas, TCGA）癌症类型与基因型组织表达数据库（Genotype-Tissue Expression, GTEx）正常组织中GSK3亚型的标准化基因表达矩阵、对应的免疫细胞富集评分（通过ssGSEA算法结合Bindea等人的基因特征集计算所得），以及经过预处理的生存分析数据（Cox回归分析结果）。 (2) 体外实验数据：经GSK3抑制剂CHIR-99021或二甲基亚砜（DMSO）对照处理的人急性髓系白血病（Acute Myeloid Leukemia, AML）THP-1细胞的凋亡与细胞周期分析原始流式细胞术文件（.fcs格式），以及包含c-Myc、切割型PARP1在内的蛋白的原始未裁剪免疫印迹（Western Blot）图像。 本数据集源自一项旨在阐明GSK3β泛癌图谱与治疗潜力的研究，其中重点聚焦于急性髓系白血病（AML）。该数据集可复用于肿瘤学与血液学领域的荟萃分析、计算验证及对比研究。