Transcriptional profiling of striatum in METH self-administered rats [miRNA]

This study aimed to identify changes in novel miRNAs and their target genes during METH self-administration and investigate their roles in METH-induced locomotion. RNA sequencing analysis revealed that mir-183-5p was upregulated in the striatum of METH self-administered rats, and target gene prediction revealed that the glucocorticoid receptor (GR) gene, Nr3c1, was a potential target gene for mir-183-5p. We confirmed that single and repeated METH administrations increased METH-induced locomotion and plasma corticosterone levels in rats.本研究旨在探究甲基苯丙胺（Methamphetamine，METH）自身给药过程中新型微小RNA（microRNAs，miRNAs）及其靶基因的表达变化，进而分析其在甲基苯丙胺诱导的运动活动中的作用。RNA测序分析表明，接受甲基苯丙胺自身给药的大鼠纹状体中，mir-183-5p表达上调；靶基因预测结果显示，糖皮质激素受体（glucocorticoid receptor，GR）基因Nr3c1为mir-183-5p的潜在靶基因。本研究证实，单次与重复给予甲基苯丙胺，均可增强大鼠的甲基苯丙胺诱导运动活性，并升高其血浆皮质酮水平。