Transcriptome analysis in primary cell models of HIV latency and cells from HIV-infected ART-suppressed individuals. Transcriptome analysis in primary cell models of HIV latency and cells from HIV-infected ART-suppressed individuals

Comparison of total RNA-seq data from ex vivo unstimulated and stimulated (with anti-CD3/CD28) cells from two primary cell models of HIV latency (resting-cell and wild-type virus models) and peripheral CD4+ T cells from HIV-infected ART-suppressed individuals (ex vivo cells). Two donors were analyzed per model. Overall design: Total RNA-seq data from ex vivo unstimulated and stimulated (with anti-CD3/CD28) primary CD4+ T cells from the resting-cell model (Lassen et al 2012), the wild-type virus model (Martins et al. 2016) and HIV-infected ART-suppressed individuals were compared (n=2 per condition).

本数据集对比了两类HIV潜伏原代细胞模型——静息细胞模型与野生型病毒模型的体外（ex vivo）未刺激及经抗CD3/CD28（anti-CD3/CD28）刺激的原代CD4+ T细胞的总RNA测序（total RNA-seq）数据，同时纳入HIV感染且接受抗逆转录病毒治疗（Antiretroviral Therapy, ART）抑制个体的体外外周血CD4+ T细胞的总RNA测序数据进行分析。各实验模型均纳入2名供体进行检测。实验设计概述：本研究对来自静息细胞模型（Lassen等，2012）、野生型病毒模型（Martins等，2016）的体外未刺激/经抗CD3/CD28刺激的原代CD4+ T细胞，以及HIV感染ART抑制个体的样本的总RNA测序数据进行了比较，各实验条件的样本量均为2（n=2）。