Spatial transcriptome analysis for squamous cell carcinoma arising from mature teratoma. Spatial transcriptome analysis for squamous cell carcinoma arising from mature teratoma

Squamous cell carcinoma arising from mature teratoma (SCC-MT) is one of the rare ovarian malignancies. Therefore, the molecular background of SCC-MT remains largely unelucidated. In this study, we performed the spatial transcriptomics for SCC-MT. Overall design: Archival fresh-frozen samples of SCC-MT were obtained from the National Cancer Center Biobank and Nagoya University. We prepared four slices from three patients with SCC-MT. Spatial transcriptomics was performed using the Visium Spatial Gene Expression Reagent Kits (10x Genomics) according to the manufacturer’s instructions.

源自成熟畸胎瘤的鳞状细胞癌（SCC-MT）是一类罕见的卵巢恶性肿瘤。目前，SCC-MT的分子背景仍在很大程度上未得到阐明。本研究针对SCC-MT开展了空间转录组学分析。
实验设计概述：本研究从国家癌症中心生物样本库（National Cancer Center Biobank）与名古屋大学获取了SCC-MT的存档新鲜冰冻样本，从3例SCC-MT患者体内制备了4张组织切片。本研究依照试剂盒生产商的操作指南，使用10x Genomics公司的Visium空间基因表达试剂盒完成了空间转录组学实验。