Urban mixtures of non-essential trace metal elements affect the behaviour and stress levels of a passerine bird in a laboratory experiment

68 male adults zebra finches were followed for 65 days in order to look for physiological and behavioral effects of 3 different mixture containing Trace Metal Elements at urban relevant does. The "Ctrl" (Control in the article) condition was exposed to bottled water only, the "ETMn" ("nTME alone" in the article) condition contained 5 mg.L-1 of As, 10 mg.L-1 of Pb and 1 mg.L-1 of Cd. The "low" ("nTME + low eTME" in the article) condition contained 5 mg.L-1 of As, 10 mg.L-1 of Pb, 1 mg.L-1 of Cd and 100 mg.L-1 of Zn, 24 mg.L-1 of Cu, 0.5 mg.L-1 of Ni. The "high" ("nTME + high eTME" in the article) condition contained 5 mg.L-1 of As, 10 mg.L-1 of Pb, 1 mg.L-1 of Cd and 200 mg.L-1 of Zn, 48 mg.L-1 of Cu, 1 mg.L-1 of Ni.本研究针对68只成年雄性斑胸草雀（zebra finch）开展了为期65天的追踪观测，旨在探究三种城市环境相关剂量的痕量金属元素（Trace Metal Elements）混合液对其产生的生理与行为效应。 本实验设置了4种暴露条件：对照组（Ctrl，原文标注为Control）仅接触瓶装水；ETMn组（原文标注为nTME alone）的混合液中含有5 mg·L⁻¹砷（As）、10 mg·L⁻¹铅（Pb）与1 mg·L⁻¹镉（Cd）；低剂量组（原文标注为nTME + low eTME）的混合液含有5 mg·L⁻¹ As、10 mg·L⁻¹ Pb、1 mg·L⁻¹ Cd，以及100 mg·L⁻¹锌（Zn）、24 mg·L⁻¹铜（Cu）、0.5 mg·L⁻¹镍（Ni）；高剂量组（原文标注为nTME + high eTME）的混合液含有5 mg·L⁻¹ As、10 mg·L⁻¹ Pb、1 mg·L⁻¹ Cd，以及200 mg·L⁻¹ Zn、48 mg·L⁻¹ Cu与1 mg·L⁻¹ Ni。