ORG.one project - Araucaria angustifolia: Whole-genome sequencing. ORG.one project - Araucaria angustifolia

“Rapid sequencing of the critically endangered Araucaria angustifolia as part of the ORG.one project https://org.one/oo”. Araucaria angustifolia (Araucariaceae) is a native species of Brazil with significant ecological, social, and economic importance. However, it has undergone extensive economic exploitation and habitat fragmentation. It is estimated that only 4.3% of the Araucaria Forest remains across its entire distribution. This has contributed to the species being classified as critically endangered on the IUCN Red List, under criterion A2cd. Despite the species' high ecological and economic value in Brazil and its concerning conservation status, little is known about its genomic constitution. The genome size of Araucaria angustifolia has been estimated at 44.7 pg (2C DNA), distributed across 26 chromosomes (2n = 26). In this project, we sequenced the genome of Araucaria angustifolia with approximately 25x coverage, using Oxford Nanopore sequencing technology. These data will support future studies on the species' evolutionary biology, population genetics, and structural and functional genomics.

本数据集系ORG.one项目（https://org.one/oo）项下针对极危物种巴西南洋杉（Araucaria angustifolia）开展的快速测序研究成果。巴西南洋杉（Araucaria angustifolia）隶属于南洋杉科（Araucariaceae），是巴西本土物种，具有极高的生态、社会与经济价值。但该物种曾遭受大规模商业开发利用，生境亦遭到严重破碎化。据估算，在其原生分布范围内，仅余4.3%的南洋杉林得以留存。这一现状使得该物种被世界自然保护联盟（IUCN）红色名录依据A2cd标准列为极危物种。尽管该物种在巴西拥有极高的生态与经济价值，且保护现状令人担忧，但学界对其基因组构成的认知仍十分有限。经估算，巴西南洋杉的基因组大小为44.7 pg（2C DNA），其染色体数目为26条（2n=26）。本项目采用牛津纳米孔（Oxford Nanopore）测序技术，以约25倍覆盖度对巴西南洋杉的基因组进行了测序。本数据集将为该物种的进化生物学、群体遗传学以及结构与功能基因组学等后续研究提供数据支撑。