Gene expression in epithelial and non-epithelial cells of renal origin. Mus musculus

We aimed to define epithelial-specific genes in the kidney. In the developing mouse kidney at E12.5 epithelial cells are restricted to the ureteric bud, while mesenchymal cells surrounding the ureteric bud are non-epithelial. The mouse renal epithelial cell line mIMCD-3 was used to represent kidney epithelia in vitro. Gene expression was analyzed using Affymetrix microarrays in ureteric bud stalks, ureteric bud tips, and mIMCD-3 cells and compared to metanephric mesenchyme. Overall design: Affymetrix Mouse Genome 430 2.0 microarrays from mouse E12.5 ureteric bud tips, ureteric bus stalks, and metanephric mesenchymes had previously been published (GEO deposition: GDS1583) (Schmidt-Ott, K. M., Yang, J., Chen, X., Wang, H., Paragas, N., Mori, K., Li, J. Y., Lu, B., Costantini, F., Schiffer, M. et al. (2005). Novel regulators of kidney development from the tips of the ureteric bud. J Am Soc Nephrol 16, 1993-2002.). We added a new microarray analysis from a subclone of mIMCD-3 cells and recalculated expression values in the complete dataset by robust multichip analysis.

本研究旨在鉴定肾脏上皮细胞特异性基因。在胚胎12.5天的发育小鼠肾脏中，上皮细胞仅局限于输尿管芽（ureteric bud）内，而环绕输尿管芽的间充质细胞均为非上皮细胞。本研究选用小鼠肾脏上皮细胞系mIMCD-3作为体外肾脏上皮细胞的模型。采用Affymetrix基因芯片（Affymetrix microarrays）分别对输尿管芽茎部、输尿管芽顶端及mIMCD-3细胞的基因表达水平进行检测，并以同期分离的后肾间充质作为对照。实验设计：此前已有针对小鼠胚胎12.5天输尿管芽顶端、输尿管芽茎部及后肾间充质的Affymetrix小鼠基因组430 2.0芯片数据发表（基因表达汇编（GEO）存档编号：GDS1583）[Schmidt-Ott, K. M.等, 2005. 源自输尿管芽顶端的肾脏发育新型调控因子. 美国肾脏病学会杂志, 16, 1993-2002.]。本研究新增了mIMCD-3细胞亚克隆的芯片分析数据，并通过稳健多芯片平均法（robust multichip analysis）重新计算了完整数据集的基因表达量。