Proteomics reveals that quinoa bioester promotes replenishing effects in epidermal tissue

The continuous search for natural products that attenuate age-related losses has increasingly gained notice; among them, those applicable for skin care have drawn major attention. The bioester generated from the Chenopodium quinoa’s oil is a natural-origin ingredient described to produce replenishing skin effects. With this as motivation, we used shotgun proteomics to study the effects of quinoa bioester on human reconstructed epidermis tridimensional cell cultures after 0, 3, 6, 12, 24, and 48 hours of exposure. Our experimental setup employed reversed-phase nano-chromatography coupled online with an Orbitrap-XL and PatternLab for proteomics as the data analysis tool. Extracted ion chromatograms were obtained as surrogates for relative peptide quantitation. Our findings spotlight proteins with increased abundancy, as compared to the untreated cell culture counterparts at same timepoints, that were related to preventing premature aging, homeostasis, tissue regeneration, protection against ultraviolet radiation and oxidative damage.

针对能够延缓衰老相关机能衰退的天然产物的持续探寻正日益受到学界关注；其中，可应用于护肤领域的天然产物更是获得了广泛瞩目。由藜麦（Chenopodium quinoa）油脂制备得到的生物酯是一种天然来源成分，据报道可发挥肌肤修护焕活的功效。基于此研究背景，我们采用鸟枪法蛋白质组学（shotgun proteomics）技术，探究了藜麦生物酯在分别暴露处理0、3、6、12、24及48小时后，对人类重建表皮三维细胞培养模型的影响。本实验采用反相纳米液相色谱与Orbitrap-XL轨道阱质谱仪在线联用的技术方案，并以蛋白质组学分析软件PatternLab作为数据分析工具。我们通过获取提取离子色谱图（extracted ion chromatograms）作为肽段相对定量的替代检测方法。本研究结果聚焦于相较于同时间点未处理对照组细胞，丰度显著上调的蛋白质；这些蛋白质与延缓皮肤早衰、维持皮肤稳态、促进组织再生以及抵御紫外线辐射和氧化损伤均密切相关。