Transcriptome profiles in the spleen of the Chinese giant salamander (Andrias davidianus) challenged with Citrobacter freundii

The Chinese giant salamander (Andrias davidianus) is one of the most important ecological breeding species with distinct characteristics and is cultured in many locations throughout China. In the present study, the transcriptome of A. davidianus spleen tissue, that had challenged with Citrobacter freundii, was analyzed using Illumina sequencing technology. The result was compared to a heathy control group. After assembly and annotation, 128,540 transcripts were generated with a median length of 349 bp. Comparative expression analysis indicated 1,995 differentially expressed genes (DEGs), 812 of which were up-regulated and 1,183 were down-regulated. Furthermore, DEGs were classified into three gene ontology categories, 535 of which were annotated to 237 KEGG pathways. Finally, six immune-related DEGs involved in the immune-related pathways were randomly selected for scrutinization. This work provides valuable data for an improved understanding the defense mechanisms of A. davidianus against bacterial pathogens at the transcriptional level. Overall design: In this study, the transcriptome of A. davidianus spleen tissue, that had challenged with Citrobacter freundii, was analyzed using Illumina sequencing technology. The result was compared to a heathy control group.Each group contained three biological replicates sample.

中国大鲵（Andrias davidianus）是具有独特特征的重要生态养殖物种，在我国多地均有养殖。本研究采用Illumina测序技术，对经弗氏柠檬酸杆菌（Citrobacter freundii）侵染的中国大鲵脾脏组织转录组（transcriptome）进行分析，并与健康对照组进行对比。经组装与注释后，共获得128540条转录本，中位长度为349 bp。差异表达分析共鉴定出1995个差异表达基因（differentially expressed genes，DEGs），其中812个基因上调表达，1183个基因下调表达。进一步地，差异表达基因被划分为3个基因本体（Gene Ontology，GO）类别，其中535个差异表达基因被注释到237条KEGG通路中。最后，随机选取6个参与免疫相关通路的免疫相关差异表达基因开展深入剖析。本研究为从转录组水平解析中国大鲵抵御细菌病原体的防御机制提供了宝贵的数据资源。整体实验设计：本研究采用Illumina测序技术，对经弗氏柠檬酸杆菌侵染的中国大鲵脾脏组织转录组进行分析，并与健康对照组进行对比，每组设置3个生物学重复样本。