Genome-wide maps of Meis1 and Hoxb13 chromatin binding in murine heart

A major factor in the progression to heart failure in humans is the inability of the adult heart to repair itself after injury. As a result, strategies to induce heart regeneration have received significant interest. We reported that transcription factors Meis1 and Hoxb13 contributes to postnatal cardiomyocyte cell cycle exit, and inhibiting these genes expression promote adult cardiomyocyte regeneration. In order to indentify the downstream targets of these transcription factors we generated ChIP-seq of mouse heart for Meis1 and Hoxb13. The results demonstrate that Meis1 and Hoxb13 act cooperatively to regulate cardiomyocyte maturation and proliferation and provide insights into the link between hyperplastic and hypertrophic growth of cardiomyocytes. Examination of Meis1 and Hoxb13 binding site in the murine heart

人类心力衰竭进展的核心诱因之一，是成体心脏在受损后无法自主修复。为此，诱导心脏再生的相关策略受到了学界广泛关注。我们此前的研究表明，转录因子Meis1与Hoxb13可促进出生后心肌细胞退出细胞周期，而抑制这两类基因的表达则能推动成体心肌细胞再生。为明确这两种转录因子的下游靶标，我们针对小鼠心脏中的Meis1和Hoxb13开展了染色质免疫共沉淀测序（ChIP-seq）实验。研究结果显示，Meis1与Hoxb13可协同调控心肌细胞的成熟与增殖，并为阐明心肌细胞增生性生长与肥厚性生长之间的关联提供了新的见解。本数据集包含小鼠心脏中Meis1与Hoxb13结合位点的相关检测分析。