Gene expression analysis in C28/I2 cells: Comparison of OENC, DPP4 Overexpression, and DPP4 Overexpression with 4,5-Dicaffeoylquinic Acid Treatment Using RNA-Seq. Gene expression analysis in C28/I2 cells: Comparison of OENC, DPP4 Overexpression, and DPP4 Overexpression with 4,5-Dicaffeoylquinic Acid Treatment Using RNA-Seq

To examine the effects of DPP4 overexpression and its modulation by 4,5-Dicaffeoylquinic acid on chondrocytes, we established C28/I2 cell lines with DPP4 overexpression (DPP4OE), control empty vector (OENC), and DPP4OE treated with 4,5-Dicaffeoylquinic acid. Gene expression profiling analysis was performed using RNA sequencing (RNA-seq) with biological replicates to investigate the global transcriptomic changes associated with these conditions. Overall design: To investigate the effects of DPP4 overexpression and its modulation by 4,5-Dicaffeoylquinic acid in C28/I2 chondrocytes, we established cell lines with DPP4 overexpression (DPP4OE), control empty vector (OENC), and DPP4OE treated with 4,5-Dicaffeoylquinic acid. Each condition was performed with three biological replicates. We then performed gene expression profiling analysis using RNA sequencing (RNA-seq) to obtain comprehensive transcriptomic data. This experimental design aims to elucidate the molecular changes associated with DPP4 overexpression and the impact of 4,5-Dicaffeoylquinic acid treatment in chondrocytes.

为探究二肽基肽酶4（DPP4）过表达及其经4,5-二咖啡酰奎宁酸（4,5-Dicaffeoylquinic acid）调控对软骨细胞的影响，我们构建了过表达DPP4的C28/I2细胞系（DPP4OE）、空载载体对照细胞系（OENC），以及经4,5-二咖啡酰奎宁酸处理的DPP4过表达细胞系。我们采用设置生物学重复的RNA测序（RNA-seq）技术开展基因表达谱分析，以探究上述各组对应的全局转录组变化。

实验整体设计：为探究DPP4过表达及其经4,5-二咖啡酰奎宁酸调控对C28/I2软骨细胞的影响，我们构建了DPP4过表达细胞系（DPP4OE）、空载载体对照细胞系（OENC），以及经4,5-二咖啡酰奎宁酸处理的DPP4过表达细胞系，每组均设置3次生物学重复。随后通过RNA测序（RNA-seq）完成基因表达谱分析，获取全面的转录组数据。本实验设计旨在阐明DPP4过表达相关的分子改变，以及4,5-二咖啡酰奎宁酸处理对软骨细胞的作用效果。