Global gastrocnemius skeletal muscle transcript data from fasted male Gcn5skm-/- (HSAcre Tg/0 x Gcn5 L2/L2) and Gcn5skm+/+ mice (HSAcre 0/0 x Gcn5 L2/L2) on a chow diet

Transcript data from gastrocnemius muscle from fasted-state male muscle-specific General Control of Amino Acid Synthesis Protein 5 knockout mice (Gcn5 skm-/- ) and wild-type mice (Gcn5skm+/+). We used microarrays to detail the effects of muscle-specific loss of GCN5 on the global program of gene expression in gastrocnemius muscle. Chow diet fed 6-months-old male mice were fasted for 5 hours, anesthetized under isoflurane, then gastrocnemius skeletal muscles were snap-frozen in liquid nitrogen for RNA extraction and RNEasy cleanup. Samples included 4 animals for each geneotype which were prepared and run on Affymetrix arrays.

本数据集来源于禁食状态下雄性肌肉特异性氨基酸合成蛋白通用调控因子5（General Control of Amino Acid Synthesis Protein 5，GCN5）基因敲除小鼠（Gcn5 skm-/-）及野生型小鼠（Gcn5skm+/+）的腓肠肌转录组数据。本研究使用微阵列（microarrays）技术，解析肌肉特异性GCN5缺失对腓肠肌全局基因表达程序的影响。实验所用小鼠为饲喂普通饲料的6月龄雄性个体，先经5小时禁食处理，再以异氟烷（isoflurane）麻醉后采集腓肠肌样本，将其置于液氮中快速冷冻，用于RNA提取及RNEasy纯化。每个基因型组包含4只小鼠，所有样本均完成制备后，在Affymetrix基因芯片平台完成检测。