Table_4_Genome-wide identification and characterization of NPF family reveals NtNPF6.13 involving in salt stress in Nicotiana tabacum.xls

Proteins of the Nitrate Transporter 1/Peptide Transporter (NPF) family transport a diverse variety of substrates, such as nitrate, peptides, hormones and chloride. In this study, a systematic analysis of the tobacco (Nicotiana tabacum) NPF family was performed in the cultivated ‘K326’. In total, 143 NtNPF genes were identified and phylogenetically classified into eight subfamilies, NPF1 to NPF8, based on the classification of NPF families in other plant species. The chromosomal locations and structures of the NtNPF genes were analyzed. The expression profiles of NtNPF genes under NaCl stress were analyzed to screen the possible NPF genes involving in chloride regulation in tobacco. Most NtNPF6 genes responded to salt stress in the roots and leaves. The expression of NtNPF6.13 was significantly down-regulated after salt stress for 12h. The chloride content was reduced in the roots of ntnpf6.13 mutant. These findings support the participation of NtNPF6.13 in chloride uptake. Several other NtNPF genes that play potential roles in chloride metabolism of tobacco require further study.

硝酸盐转运蛋白1/肽转运蛋白（Nitrate Transporter 1/Peptide Transporter，NPF）家族蛋白可转运多种底物，涵盖硝酸盐、肽类、激素及氯化物等。本研究以栽培品种‘K326’烟草（Nicotiana tabacum）为材料，对其NPF家族开展系统分析。研究共鉴定得到143个烟草NPF（NtNPF）基因，参照其他植物物种NPF家族的分类标准，将其系统划分为NPF1至NPF8共8个亚家族。随后分析了NtNPF基因的染色体定位与基因结构。为筛选参与烟草氯化物调控的潜在NPF基因，本研究解析了NtNPF基因在NaCl胁迫下的表达谱。结果表明，多数NtNPF6亚家族基因在烟草根与叶中均响应盐胁迫；其中NtNPF6.13的表达在盐胁迫处理12小时后显著下调。ntnpf6.13突变体植株的根系中氯化物含量降低，上述发现证实NtNPF6.13参与了氯化物的吸收过程。另有若干其他NtNPF基因在烟草氯化物代谢中发挥潜在作用，有待进一步研究。