Antibacterial cross-streak activity by actinomycetes: ActinoData project

Photographs taken of cross-streak agar plates with an antibiotic-producing actinomycete strain streaked in the middle of the plate, incubated for 7 days at 30 degrees Celsius (optimal growth temperature of actinomycetes), and the bacterial test strains (pathogens) streaked from the outer edge of the agar plate up to the actinomycete growth in the middle. Plates are incubated at 37 degrees Celsius (optimal growth temperature of the pathogenic strains) for 24hrs and inspected for activity. Gaps between the bacterial streak and the central growth streak serve as an indicator that the actinomycete produced a bioactive compound that inhibited the growth of the bacterium. The test bacterial strains (pathogens) streaked on the plate are in the following order:On the left, top to bottom:<i>Acinetobacter baumannii</i> ATCC 19606; <i>Klebsiella quasipneumoniae</i> ATCC 700603; <i>Staphylococcus aureus</i> ATCC 33591 (MRSA); <i>Enterococcus faecalis</i> ATCC 29212; and <i>Enterococcus faecalis</i> ATCC 51299 (VanB)On the right, top to bottom:<i>Acinetobacter baumannii</i> ATCC 1605; <i>Enterobacter cloacae</i> ATCC 1143; <i>Escherichia coli</i> ATCC 25922; <i>Pseudomonas aeruginosa</i> ATCC 27853; and <i>Staphylococcus aureus</i> ATCC 29213<br><br>Images are labelled as follow:actinomycete strain name_media type_Bacteria_agar<br>The actinomycete strain name will vary, while the media type could be M19 (g/L: 20 mannitol, 10 casamino acids, 20 peptone, 20 bacteriological agar, pH7.0) or ISP2 (g/L: 10 malt extract, 4 yeast extract, 4 glucose, 20 bacteriological agar, pH7.0). 'Bacteria' denotes that the analysis was performed on bacterial test strains, while 'agar' refers the the side of the plate visualized in the photograph.

本数据集包含的图像为十字划线琼脂平板的拍摄照片，具体制备与实验流程如下：将产抗生素放线菌菌株划线接种于平板中央，于30℃（放线菌最适生长温度）培养7天；随后将受试细菌菌株（病原菌）从平板外缘向中央的放线菌生长区域划线。将平板置于37℃（受试病原菌的最适生长温度）培养24小时后，观察其抑菌活性。当受试细菌划线区域与中央放线菌生长区域之间出现抑菌间隙时，即表明该放线菌产生了可抑制该细菌生长的生物活性化合物。

平板上的受试细菌菌株排布顺序如下：
左侧（从上至下）：鲍氏不动杆菌（*Acinetobacter baumannii*）ATCC 19606；类肺炎克雷伯菌（*Klebsiella quasipneumoniae*）ATCC 700603；金黄色葡萄球菌（*Staphylococcus aureus*）ATCC 33591（耐甲氧西林金黄色葡萄球菌，MRSA）；粪肠球菌（*Enterococcus faecalis*）ATCC 29212；粪肠球菌（*Enterococcus faecalis*）ATCC 51299（VanB型耐万古霉素菌株）
右侧（从上至下）：鲍氏不动杆菌（*Acinetobacter baumannii*）ATCC 1605；阴沟肠杆菌（*Enterobacter cloacae*）ATCC 1143；大肠埃希菌（*Escherichia coli*）ATCC 25922；铜绿假单胞菌（*Pseudomonas aeruginosa*）ATCC 27853；金黄色葡萄球菌（*Staphylococcus aureus*）ATCC 29213

图像命名格式为：放线菌菌株名_培养基类型_细菌_琼脂侧。其中放线菌菌株名会随样本不同而变化，培养基类型可为M19（配方：g/L：甘露醇20、酪蛋白氨基酸10、蛋白胨20、细菌琼脂20，pH7.0）或ISP2（配方：g/L：麦芽提取物10、酵母提取物4、葡萄糖4、细菌琼脂20，pH7.0）。命名字段中的“细菌”表示本次分析对象为受试细菌菌株，“琼脂侧”指照片拍摄的平板琼脂面。