Additional file 1: of Viral diversity is an obligate consideration in CRISPR/Cas9 designs for targeting the HIV reservoir

Table S1. (A) Correlation between predicted activity and target site conservation. (B) Correlation between measured and predicted activity. (C) Correlation between measured activity and target site prevalence. Table S2. List of highly conserved, subtype-specific triplet/paired gRNAs. Table S3. Analysis of the number of guides needed to target all available LANL sequences for LTR, gag, and pol for group M and subtypes A–C. Table S4. GFP knockdown with candidate guides tested using fluorescent reporter. Table S5. Sequences used in intra-host analysis. Table S6. Guides from globally conserved list (using LANL sequences) that have matches in patient sequence. (XLSX 59 kb)

表S1。（A）预测活性与靶位点保守性的相关性；（B）实测活性与预测活性的相关性；（C）实测活性与靶位点流行率的相关性。
表S2：高度保守且亚型特异性的三联体/配对gRNA（guide RNA）列表。
表S3：针对M组及A~C亚型的LTR（长末端重复序列）、gag与pol基因，靶向LANL所有可用序列所需向导RNA数量的分析。
表S4：采用荧光报告基因检测候选向导RNA的GFP敲降实验结果。
表S5：宿主内分析所用序列列表。
表S6：源自基于LANL序列构建的全局保守列表、且在患者序列中存在匹配位点的向导RNA。
（XLSX格式，59千字节）